diff --git a/gtk/theme/Adwaita/_common.scss b/gtk/theme/Adwaita/_common.scss
index be3ac05761..b1dacb9f84 100644
--- a/gtk/theme/Adwaita/_common.scss
+++ b/gtk/theme/Adwaita/_common.scss
@@ -671,6 +671,10 @@ toolbar.inline-toolbar toolbutton:backdrop {
   &:only-child > button.flat { @extend %linked:only-child; }
 }
 
+toolbar separator {
+  background: none;
+}
+
 %linked_middle {
   border-radius: 0;
   border-right-style: none;
diff --git a/gtk/theme/Adwaita/gtk-contained-dark.css b/gtk/theme/Adwaita/gtk-contained-dark.css
index 8d5acf2bc6..36165e5814 100644
--- a/gtk/theme/Adwaita/gtk-contained-dark.css
+++ b/gtk/theme/Adwaita/gtk-contained-dark.css
@@ -1111,6 +1111,10 @@ button, headerbar button.titlebutton,
     .inline-toolbar toolbutton > button:backdrop:insensitive:active > .label, .inline-toolbar toolbutton > button:backdrop:insensitive:checked > .label {
       color: inherit; }
 
+toolbar separator, .inline-toolbar separator, searchbar separator,
+.location-bar separator {
+  background: none; }
+
 toolbar.inline-toolbar toolbutton > button.flat, .inline-toolbar toolbutton > button.flat, searchbar.inline-toolbar toolbutton > button.flat,
 .inline-toolbar.location-bar toolbutton > button.flat, toolbar.inline-toolbar toolbutton > button.sidebar-button, .inline-toolbar toolbutton > button.sidebar-button, searchbar.inline-toolbar toolbutton > button.sidebar-button,
 .inline-toolbar.location-bar toolbutton > button.sidebar-button, .inline-toolbar headerbar toolbutton > button.titlebutton, headerbar .inline-toolbar toolbutton > button.titlebutton,
diff --git a/gtk/theme/Adwaita/gtk-contained.css b/gtk/theme/Adwaita/gtk-contained.css
index 3086945c89..2f602c1f87 100644
--- a/gtk/theme/Adwaita/gtk-contained.css
+++ b/gtk/theme/Adwaita/gtk-contained.css
@@ -1111,6 +1111,10 @@ button, headerbar button.titlebutton,
     .inline-toolbar toolbutton > button:backdrop:insensitive:active > .label, .inline-toolbar toolbutton > button:backdrop:insensitive:checked > .label {
       color: inherit; }
 
+toolbar separator, .inline-toolbar separator, searchbar separator,
+.location-bar separator {
+  background: none; }
+
 toolbar.inline-toolbar toolbutton > button.flat, .inline-toolbar toolbutton > button.flat, searchbar.inline-toolbar toolbutton > button.flat,
 .inline-toolbar.location-bar toolbutton > button.flat, toolbar.inline-toolbar toolbutton > button.sidebar-button, .inline-toolbar toolbutton > button.sidebar-button, searchbar.inline-toolbar toolbutton > button.sidebar-button,
 .inline-toolbar.location-bar toolbutton > button.sidebar-button, .inline-toolbar headerbar toolbutton > button.titlebutton, headerbar .inline-toolbar toolbutton > button.titlebutton,